Journal of Experimental and Clinical SurgeryJournal of Experimental and Clinical Surgery2070-478X2409-143XVoronezh State Medical University103210.18499/2070-478X-2017-10-4-293-303UnknownThe Algorithm For Obtaining Referential Meanings Of Biochemical And Complete Blood Tests Coagulogram In Wistar Male Rats At Blood Sampling From Carotid ArteryKinzerskiyA A<p>postgraduate student of Department of the General surgery</p>kinzerskij@mail.ruhttps://orcid.org/0000-0001-5749-1873KorzhukM S<p>MD, Head of Department of the General surgery</p>kinzerskij@mail.ruhttps://orcid.org/0000-0002-4579-2027DolgichV T<p>MD, Honoured Scientist of the Russian Federation, Head of Department of the Pathophysiology including Clinical Pathophysiology Course of Omsk State Medical University</p>kinzerskij@mail.ruhttps://orcid.org/0000-0001-9034-4912Omsk State Medical University of the Russian Federation Ministry of Health311220171042933031506201716062017Copyright © , KINZERSKIY A.A., KORZHUK M.S., DOLGICH V.T.<p>Background. At present time there are few works throwing some light on the details of solving a problem in a field of experimental medicine by the "how to do it" principle which after having studied them give readers a good grasp of reproducing the offered technique on their own. It also concerns blood sampling with the aim of assessing certain indicators at small laboratory animals.</p>
<p>Objectives. To work out the technique of carotid artery catheterization for detecting the referential meanings of biochemical and complete blood tests coagulogram on Wistar male rats. To define more precisely the obtained values by the bootstrap method and to compare them with the data to have published before.</p>
<p>Materials and methods. The research was conducted on Wistar male rats (n=42) weighing 270-380 g. The access to the left carotid artery was implemented with taking into account some anatomic features of an animal being anesthetized with tiletamin/zolazepam (20-40 mg/kg intramuscularly) + xylazine (5-10 mg / kg. intramuscularly). Statistical treatment has been conducted in the programming language R with using the statistical packages "boot", "car", "sm", "coin", "VIM" and "mice". The referential meanings have been defined by the bootstrap method.</p>
<p>Comparing with literature data was executed by the permutational test with p-level correction for multiple comparisons by using the Benjamini-Yekutieli method, the level was accepted as equal 0,05.</p>
<p>Results and Discussion. The rational access to a carotid artery and its catheterization way have been developed. The referential meanings of biochemical and complete blood tests coagulogram were developed and defined by using the bootstrap method on Wistar male rats. While comparing the indicators to have received earlier from Wistar male rats by other researchers, the statistically significant differences were received in all indicators, except the quantity of leucocytes, thrombocytes, and indicators of hematocrit and total bilirubin. The received differences are likely to be connected with different climatic conditions, the character of anesthesia care, accessing to blood sampling, and wide range of the compared rats age. Clinically differences are insignificant.</p>
<p>Conclusion. The developed algorithm for obtaining referential meanings of blood tests samplings from rats can be considered to be rational and reproduced and the received indicators values should be considered to be referential.</p>Wistar male rats, referential meanings, carotid artery, complete blood tests, biochemical blood tests, coagulogram, bootstrap method, permutation testкрысы самцы Wistarреференсные значениясонная артерияобщий анализ кровибиохимический анализ кровикоагулограммабутстрептест пермутации[Kinzerskii A.A., Petrova Iu.A, Korzhuk M.S., Dolgikh V.T. Normal'nye znacheniia obshchego, biokhimicheskogo analiza krovi i koagulogrammy krys-samtsov linii Wistar [The normal values of biochemical and complete blood tests, and coagulogram of Wistar male rats] The certificate of state registration of the database No. 2017620486. Bull. 2017; 5:1-2. http://www1.fips.ru/wps/PA_FipsPub/res/Doc/PrEVM/RUNWDB/000/002/017/620/486/2017620486-00001/DOCUMENT.PDF. (in Russ.)][Korzhuk M. S., Kozlov K. K., Tkachev A. G., Vial'tsin A. S., Rubanik V. Iu. Usovershenstvovannyi sposob vydeleniia sosudov i ostanovki krovotecheniia [The improved method of separating vessels and bleeding control] Herald of Surgery by GrekovI.I..2015;6:52 – 55. (in Russ.)][Mastitskii S.E., Shitikov V.K. Statisticheskii analiz i vizualizatsiia dannykh s pomoshch'iu R [Statistical analysis and visualization of data using R] Heidelberg-London-Tol'yatti. 2014; 401. http://www.ievbras.ru/ecostat/Kiril/R/Mastitsky%20and%20Shitikov%202014.pdf. (in Russ.)][Shitikov V.K., Rozenberg G.S. Randomizatsiia i butstrep: statisticheskii analiz v biologii i ekologii s ispol'zovaniem R [Randomization and bootstrap: statistical analysis in biology and ecology using R]Tol'yatti.: “Kassandra” Publishers. 2013; 314. http://www.ievbras.ru/ecostat/Kiril/Article/A32/Starb.pdf][Animal Resources Centre. Male and female Wistar and Sprague-Dawley rats from 3 to 12 weeks held at the Animal Resources Centre. - Canning Vale, Australia. 2017. http://www.arc.wa.gov.au/?page_id=125][Clifford C.B., Giknis M.L.A. Clinical laboratory parameters for Crl:WI(Han) rats. Wilmington.: “Charles river” Publishers. 2008; 17. http://www.criver.com/files/pdfs/rms/wistarhan/rm_rm_r_wistar_han_clin_lab_parameters_08.aspx][Kabacoff R. I. R in action: Data analysis and graphics with R. Shelter Island.: “Manning” Publishers. 2011; 474. http://kek.ksu.ru/eos/DataMining/1379968983.pdf.][Oruganti M. and Gaidhani S. Routine bleeding techniques in laboratory rodents. IJPSR. 2011; 2(3):516 524. http://ijpsr.com/bft-article/routine-bleeding-techniques-in laboratory rodents/?view=fulltext][Parasuraman S., Raveendran R., Kesavan R. Blood sample collection in small laboratory animals. J Pharmacol Pharmacother. 2010; 1(2):87–93. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3043327/][Seibel J., Bodie´ K., Weber S., Bury D., Kron M. and Blaich G. Comparison of haematology, coagulation and clinical chemistry parameters in blood samples from the sublingual vein and vena cava in Sprague–Dawley rats. Lab Anim. 2010; 44(4):344-51. http://journals.sagepub.com/doi/pdf/10.1258/la.2010.009049.][Sengupta P. The laboratory rat: relating its age with human's. Int J Prev Med. 2013; 4(6):624-30. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733029/][Sengupta P. The laboratory rat: relating its age with human's. Int J Prev Med. 2013; 4(6):624-30. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733029]