Journal of Experimental and Clinical SurgeryJournal of Experimental and Clinical Surgery2070-478X2409-143XVoronezh State Medical University111210.18499/2070-478X-2017-10-4-265-268UnknownThe role of bacteriophages in the complex treatment of peritonitis of appendicular origin (experimental study)MokhovEvgeny Mikhailovich<p>Doctor of Medical Science, Professor, Head of the Department of General Surgery</p>koch2006@mail.ruhttps://orcid.org/0000-0003-3099-0132MorozovArtem Mikhailovich<p>Assistant of the Department of General Surgery</p>ammorozovv@gmail.comhttps://orcid.org/0000-0003-4213-5379KadykovVictor Alekseevich<p>Candidate of Medical Sciences., Associate Professor of the Department of General Surgery</p>koch2006@mail.ruhttps://orcid.org/0000-0002-7516-3467AskerovElshad Magomedovich<p>Candidate of Medical Sciences., Associate Professor of the Department of General Surgery</p>koch2006@mail.ruSerovaNina Evgenievna<p>Candidate of Medical Sciences., Associate Professor of the Department of Pathological Anatomy</p>koch2006@mail.ruTver State Medical University311220171042652681210201716102017Copyright © , Mokhov E.M., Morozov A.M., Kadykov V.A., Askerov E.M., Serova N.E.<p>Relevance: with the management of laparoscopic methods of surgical treatment of acute appendicitis, the problem of the occurrence of early postoperative complications has not changed at present, which makes it necessary to carry out preventive measures.</p>
<p>Objective: to study the possibility of improving the results of surgical treatment of peritonitis, the most frequent complication of acute appendicitis, by reducing the number of postoperative complications of infectious genesis.</p>
<p>Methods: Studies were performed on 47 non-linear white rats weighing 200-250 grams by modelling peritonitis using the biological model as an example, followed by antibiotic therapy and combination therapy using bacteriophages. To model peritonitis, the infection of the abdominal cavity with the E. coli strain 25922 was used. The laboratory animals were divided into 3 groups: the first group was control group, the second group received treatment in the form of a single intraperitoneal injection of Cefipime, the third group received treatment as a single intraperitoneal injection of the Sextapage.</p>
<p>Results: the evaluation of the methods was carried out on the basis of the study of the clinical picture of the course of peritonitis in experimental animals, the data of pathomorphological and histological examination of the sectional peritoneum. As a result of experimental studies, there was no significant difference in the methods of perioperative prevention of complications of acute appendicitis, which makes it possible to perform monotherapy with bacteriophages. Phages in comparison with antibiotics have no less therapeutic efficacy.</p>
<p>Conclusions: Thus, our experimental studies revealed completely satisfactory results of monotherapy of experimental peritonitis with a bacteriophage. According to the morphological data, the inflammatory process in the abdominal cavity is stopped by the phage fast enough and there is a tendency for a faster fading of the inflammation than in the treatment with antibiotics.</p>acute appendicitis, peritonitis, bacteriophages, perioperative bacteriophage therapyострый аппендицит, перитонит, бактериофаги, периоперационная бактериофаготерапия[1. Styazhkina S.N., Salavatullin A.V., Kuznecov S.F., Aleksandrov A.YU. Problemnye voprosy hirurgicheskogo lecheniya ostrogo appendicita i ego oslozhnenij. Sovremennye tendencii razvitiya nauki i tekhnologij. 2016, № 3-2,55-57][2. Morozov A.M., Zhukova D.A. Rezul'taty lecheniya ostrogo appendicita // Materialy 61-j nauchnoj konferencii studentov «Molodyozh', nauka, medicina» , Tver', 23 aprelya 2015 g., RIC TGMA 2015g.,138-141.][3. Aslanov B.I., Zueva L.P., Kaftyreva L.A. Racional'noe primenenie bakteriofagov v lechebnoj i protivoehpidemicheskoj praktike // Federal'nye klinicheskie rekomendacii – Moskva, 2014 g. 39 p.][4. Briusov PG., Zubritskii VF., Islamov RN., Nizovoĭ AV., Fominykh EM. Phagoprophylaxis and bacteriophage treatment of surgical infections // Voen Med Zh. - 2011 - 332(4): 34-9.][5. Kutateladze M., Adamia R. Bacteriophages as potential new therapeutics to replace or supplement antibiotics // Trends Biotechnol -2010- 28(12): 591-5.][6. Rios AC., Moutinho CG., Pinto FC., Del Fiol FS., Jozala A., Chaud MV., Vila MM., Teixeira JA., Balcão VM. Alternatives to overcoming bacterial resistances: State-of-the-art // Microbiol Res - 2016 - 191:51-80.]