Journal of Experimental and Clinical SurgeryJournal of Experimental and Clinical Surgery2070-478X2409-143XVoronezh State Medical University32610.18499/2070-478X-2011-4-4-764-772UnknownPilot clinical trial of tissue engineered constructs consisting of autologous stromal cells from adipose tissue in patients with bone tissue deficiency in the field of the alveolar process of maxilla and mandibleauthor@vestnik-surgery.comauthor@vestnik-surgery.comauthor@vestnik-surgery.comauthor@vestnik-surgery.comauthor@vestnik-surgery.comauthor@vestnik-surgery.comauthor@vestnik-surgery.comauthor@vestnik-surgery.com24122011447647722904201629042016Copyright © 2011, ., ., ., ., ., ., ., .2011Despite the progress in conventional methods of maxillofacial surgery, which are based on the substitution of bone defects, alternative methods are being intensively developed. Recent studies focused on the use of a variety of matrices either inorganic or organic (autologous bone) have shown the limitations of these materials: inorganic matrices do not possess sufficient osteoinductive capacity and autologous bone cannot be obtained in large amounts. The presented results of the pilot clinical trial point to the fact that the introduction of tissue engineered constructs consisting of stromal cells from adipose tissue and osteoinductive materials have a beneficial effect on wound healing and formation of new bone tissue.Maxillofacial surgeryautologous stromal cells from adipose tissuetissue engineeringsinus liftingbone augmentation.Челюстно-лицевая хирургияаутологичные стромалъные клетки из жировой тканитканевая инженериясинус-лифтингостеопластика.[1. Agapov V.S., Asnina S.A., Volozhin A.I., Mal'ko E.V., Khaled Al' Khindi. Primenenie preparatov kolapol i gidroksiapol dlia zapolneniia polostei posle udaleniia radikuliarnykh kist [Use of medicines колапол and гидроксиапол for filling of cavities after removal the radikulyarnykh of cysts]. Meditsinskaia konsul'tatsiia. 1996; 3: 44-45.][2. Agapov V.S., Asnina S.A., Volozhin A.I., Belozerov M.N., Mazur L.G., Mal'ko E.V. Analiz blizhaishikh i otdalennykh rezul'tatov primeneniia osteoplasticheskikh materialov gidroksiapol i kolapol v poliklinicheskoi khirurgicheskoi praktike. Iubileinyi spisok rabot, posviashchennyi 60-letiiu kafedry gospital'noi khirurgicheskoi stomatologii i cheliustno-litsevoi khirurgii [The analysis of the immediate and remote results of use of osteoplastic materials гидроксиапол and колапол in polyclinic surgical practice. The anniversary list of works devoted to the 60 anniversary of department of hospital surgical stomatology and maxillofacial surgery]. Moskva: MMSI. 1998; 25-27.][3. Belozerov M.N. Otsenka osteoplasticheskikh svoistv razlichnykh biokompozitsionnykh materialov dlia zapolneniia defektov cheliustei (eksperimental'no-klinicheskoe issledovanie) [Assessment of osteoplastic properties of various biocomposite materials for filling of defects of jaws (experimental clinical trial)]. Dis. …kand. med. nauk. M. 2004; 147.][4. Volozhin A.I., Popov V.K., Kraslov A.P. i dr. Fiziko-mekhanicheskie i morfologicheskie kharakteristiki novykh kompozitov na osnove sverkhmolekuliarnogo polietilena i gidroksiapatita [Physicomechanical and morphological characteristics of new composites on the basis of supermolecular polyethylene and a hydroxyapatite]. «Novoe v stomatologii». 1999; 8: 35-43.][5. Kulakov A.A., Gol'dshtein D.V., Grigorian A.S. i dr. Klinicheskoe issledovanie effektivnosti primeneniia kombinirovannogo kletochnogo transplantata na osnove mul'tipotentnykh mezenkhimal'nykh stromal'nykh kletok zhirovoi tkani u patsientov s vyrazhennym defitsitom kostnoi tkani v oblasti verkhnei i nizhnei cheliusti [Clinical trial of efficiency of application of the combined cellular transplant on a basis the multipotentnykh mesenchymal the stromalnykh of cells of fatty tissue at patients with the expressed deficit of a bone tissue in the upper and lower jaw]. Kletochnye tekhnologii v biologii i meditsine. 2008; 4: 206-210.][6. Drobyshev A.Iu. Eksperimental'noe obosnovanie i prakticheskoe primenenie otechestvennykh biokompozitnykh materialov pri kostnovostanovitel'nykh operatsiiakh na cheliustiakh [Experimental justification and practical use of domestic biocomposite materials at the kostnovostanovitelnykh jaw operations]. Diss. Dok-ra med. nauk. M. 2001; 237.][7. Maksimovskii Iu.M., Churkova T.D., Volozhin A.I. Novyi otechestvennyi preparat gidroksiapol pri khirurgicheskom lechenii parodontita [New domestic medicine hydroxiapol at surgical treatment of a periodontal disease]. Zubovrachebnyi vestnik. 1993; 3: 19-22.][8. Rubina K.A., Kalinina N.I., Efimenko A., Lopatina T., Melikhova V., Tsokolaeva Z.I., Sysoeva V.Iu., Tkachuk V.A., Parfenova E.V. Mekhanizm stimuliatsii angiogeneza v ishemizirovannom miokarde s pomoshch'iu stromal'nykh kletok zhirovoi tkani [The mechanism of stimulation of angiogenesis in an ischemic myocardium with the help the stromalnykh of cells of fatty tissue]. Kardiologiia. 2010; 2: 58-66.][9. Savel'ev V.I., Kornilov N.V., Kalinin A.V., Bulatov A.A. Tekhnologii izgotovleniia i eksperimental'no-klinicheskogo primeneniia demineralizovannykh kostnykh transplantatov [Manufacturing techniques and an experimental clinical use of the demineralized osteal grafts]. Simpozium po problemam tkanevykh bankov s mezhdunarodnym uchastiem. Sankt-Peterburg: Rossiia. 2001; 27.][10. Chiapasco M., Drusati R., Ronchi P. Clin Oral Implants Reg. 2007; 18: 1: 74-85.][11. Dudas J.R., Marra K.G., Cooper G.M. et al. The osteogenic potential of adipose-derived stem cells for the repair of rabbit calvarial defects. Ann. Plast. Surg. 2006; 56: 543-548.][12. Fang B., Song Y., Lin Q. et al. Pediatr Transplant. 2007; 11: 7: 814-817.][13. Gronthos S., Franklin D.M., Leddy H.A. et al. Surface protein characterization of human adipose tissue-derived stromal cells. J. Cell Physiol. 2001; 189: 54-63.][14. Kinnaird T., Stabile E., Burnett M.S. et al. Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms. Circ Res. 2004; 94: 678-685.][15. Lendeskel S., Jödicke A., Christophis P. et al. J. Craniomaxillofac Surg. 2004; 32: 6: 370-373. Li X., Jin L., Balian G. et al. Biomaterials. 2006; 27: 11: 2426-2433.][17. Martin-Rendon E., Hale S.J., Ryan D. et al. Transcriptional profiling of human cord blood CD133+ and cultured bone marrow mesenchymal stem cells in response to hypoxia. Stem Cells. 2007; 25: 1003-1012.][18. Ohnishi S., Yasuda T., Kitamura S. et al. Effect of hypoxia on gene expression of bone marrow-derived mesenchymal stem cells and mononuclear cells. Stem cells. 2007; 25: 1166-1177.][19. Rah D.K. Art of replacing Craniofacial bone defects. Yonsi Med. J. 2000; 41: 756-765.][20. Rehman J., Traktuev D., Li J. et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004; 109: 1292-1298.][21. Rubina K., Kalinina N., Efimenko A., Lopatina T., Melikhova V., Tsokolaeva Z., Sysoeva V., Tkachuk V., Parfyonova Y. Adipose Stromal Cells Stimulate Angiogenesis via Promoting Progenitor Cell Differentiation, Secretion of Angiogenic Factors and Enhancing Vessel Maturation. Tissue Eng. Part A. 2009; 15: 8: 2039-2050.][22. Sadat S., Gehmert S., Song Y.-H. et al. The cardioprotective effect of mesenchymal stem cells is mediated by IGF-I and VEGF. Biochem Biophys Res Com. 2007; 363, 674.][23. Stashower M., Smith K., Willams J., Skelton H. Dermatol Surg. 1999; 25: 5: 945][24. Weinzierl K., Yemprich A., Frerich B.J. Craniomaxillofac Surg. 2006; 34: 8: 466-471.][25. Zuk P.A., Zhu M., Ashjian P., De Ugarte D.A., Huang J.I., Mizuno H., Alfonso Z.C., Fraser J.K., Benhaim P., Hedrick M.H. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13: 4279]