Journal of Experimental and Clinical SurgeryJournal of Experimental and Clinical Surgery2070-478X2409-143XVoronezh State Medical University8810.18499/2070-478X-2015-8-4-369-372UnknownPathology and mineral composition of bone in the early stages of experimental osteomyelitismail@vestnik-surgery.commail@vestnik-surgery.commedic_82@mail.ruIzhevsk State Medical Academy, 426034, Izhevsk, Udmurt republic, Kommunarov Street, 28124122015843693722604201626042016Copyright © 2015, ., ., .2015<div style="left: 108.661px; top: 929.159px; font-size: 15px; font-family: serif; transform: scaleX(1.01439);" data-canvas-width="63.315000000000005">Relevance</div><div style="left: 171.961px; top: 929.159px; font-size: 15px; font-family: serif; transform: scaleX(1.04021);" data-canvas-width="729.4319999999997">Study of pathogenetic patterns and the nature of the structural changes in the bone tissue in the early stages of acute</div><div style="left: 108.661px; top: 947.159px; font-size: 15px; font-family: serif; transform: scaleX(1.04191);" data-canvas-width="792.7380000000002">osteomyelitis is relevant in terms of increased knowledge about mechanisms of development of complications in patients with</div><div style="left: 108.661px; top: 965.159px; font-size: 15px; font-family: serif; transform: scaleX(1.04024);" data-canvas-width="792.7304999999999">orthopedic and rehabilitation of the complex. The purpose of the work comprehensive assessment of pathological changes and</div><div style="left: 108.661px; top: 983.159px; font-size: 15px; font-family: serif; transform: scaleX(0.997138);" data-canvas-width="657.7289999999999">features of the mineral composition of bone tissue in the early stages of acute osteomyelitis in the experiment.</div><div style="left: 108.661px; top: 1001.16px; font-size: 15px; font-family: serif; transform: scaleX(1.04603);" data-canvas-width="142.8705">Materials and methods</div><div style="left: 251.532px; top: 1001.16px; font-size: 15px; font-family: serif; transform: scaleX(1.01338);" data-canvas-width="649.8660000000001">The experiments were performed on 35 rabbits of both sexes, in accordance with bioethical requirements.</div><div style="left: 108.661px; top: 1019.16px; font-size: 15px; font-family: serif; transform: scaleX(1.01203);" data-canvas-width="792.7409999999996">Acute hematogenous osteomyelitis modeled by introducing a culture of Staphylococcus aureus in the medullary canal of the tibia.</div><div style="left: 108.661px; top: 1037.16px; font-size: 15px; font-family: serif; transform: scaleX(0.995565);" data-canvas-width="792.7184999999998">All animals were obtained from a short-term experience under ether anesthesia by air embolism after 30 min, 6, 12, 24 and 48 hours</div><div style="left: 108.661px; top: 1055.16px; font-size: 15px; font-family: serif; transform: scaleX(1.02503);" data-canvas-width="792.723">after administration Staphylococcus aureus culture. Information on the timing of the experiment by light, electron (scanning and</div><div style="left: 108.661px; top: 1073.16px; font-size: 15px; font-family: serif; transform: scaleX(1.00604);" data-canvas-width="792.729">transmission) microscopy and X-ray electron microprobe analysis studies especially pathomorphology and mineral composition of</div><div style="left: 108.661px; top: 1091.16px; font-size: 15px; font-family: serif; transform: scaleX(1.00042);" data-canvas-width="70.83">bone tissue.</div><div style="left: 108.661px; top: 1109.16px; font-size: 15px; font-family: serif; transform: scaleX(1.06803);" data-canvas-width="182.38349999999997">Results and their discussion</div><div style="left: 292.936px; top: 1109.16px; font-size: 15px; font-family: serif; transform: scaleX(1.04434);" data-canvas-width="608.4494999999998">During the first 30 minutes of the experiment micro- and ultramicroscopic changes in bone were</div><div style="left: 108.661px; top: 1127.16px; font-size: 15px; font-family: serif; transform: scaleX(0.989985);" data-canvas-width="792.7305">minimal: preserved bone rods with small groups of red blood cells, and the mineral content of bones was characterized by a decrease</div><div style="left: 108.661px; top: 1145.16px; font-size: 15px; font-family: serif; transform: scaleX(1.03506);" data-canvas-width="792.7335">in the volume fraction of sodium. At the 6 hour experiment there was also no significant structural rearrangements of bone, but</div><div style="left: 108.661px; top: 1163.16px; font-size: 15px; font-family: serif; transform: scaleX(1.03852);" data-canvas-width="792.7365000000001">there was a further reduction in the concentration of sodium and magnesium, and increase the volume fraction of sulfur. At the</div><div style="left: 108.661px; top: 1181.16px; font-size: 15px; font-family: serif; transform: scaleX(1.03512);" data-canvas-width="792.7289999999998">same time, this period of the experiment was an increase in volume fraction of calcium and phosphorus. Morphological picture</div><div style="left: 108.661px; top: 1181.16px; font-size: 15px; font-family: serif; transform: scaleX(1.03512);" data-canvas-width="792.7289999999998"><div style="left: 118.11px; top: 115.159px; font-size: 15px; font-family: serif; transform: scaleX(1.02091);" data-canvas-width="792.7365000000001">in the next 12 hours of the experiment were characterized by the beginning of the destructive processes in the bone marrow, with</div><div style="left: 118.11px; top: 133.159px; font-size: 15px; font-family: serif; transform: scaleX(1.00561);" data-canvas-width="792.7364999999998">what is likely to involve some adjustment of mineral metabolism of bone tissue. There was a sharp increase in the concentration of</div><div style="left: 118.11px; top: 151.159px; font-size: 15px; font-family: serif; transform: scaleX(1.00735);" data-canvas-width="792.735">sodium while maintaining high values of volume fractions of intraosseous calcium, phosphorus and sulfur, reflecting the increased</div><div style="left: 118.11px; top: 169.159px; font-size: 15px; font-family: serif; transform: scaleX(0.975237);" data-canvas-width="792.7380000000003">mineralization. By the end of first day of the experiment to develop the process of destruction of bone tissue, which were accompanied</div><div style="left: 118.11px; top: 187.159px; font-size: 15px; font-family: serif; transform: scaleX(1.00093);" data-canvas-width="792.7379999999999">by demineralization. Second day from start of the experiment were activated processes of bone formation, and the demineralization</div><div style="left: 118.11px; top: 205.159px; font-size: 15px; font-family: serif; transform: scaleX(1.01516);" data-canvas-width="57.915">persisted.</div><div style="left: 118.11px; top: 223.159px; font-size: 15px; font-family: serif; transform: scaleX(1.03267);" data-canvas-width="80.44500000000001">Conclusions</div><div style="left: 199.11px; top: 223.159px; font-size: 15px; font-family: serif; transform: scaleX(1.01606);" data-canvas-width="711.735">Thus, within one day of the experiment structural rearrangements missing bone, and its mineral composition change</div><div style="left: 118.11px; top: 241.159px; font-size: 15px; font-family: serif; transform: scaleX(0.974322);" data-canvas-width="792.7410000000002">associated with the adaptation process active in response to inflammation in the medullary canal. Second day experience of developing</div><div style="left: 118.11px; top: 259.159px; font-size: 15px; font-family: serif; transform: scaleX(1.01456);" data-canvas-width="792.7244999999999">bone destruction is accompanied by phenomena of osteoporosis, along with activation of osteosynthesis. Period for first day from</div><div style="left: 118.11px; top: 277.159px; font-size: 15px; font-family: serif; transform: scaleX(1.00339);" data-canvas-width="757.9590000000001">entering the pathogen can be considered as the most favorable in terms of prevention of subsequent orthopedic complications.</div><div style="left: 118.11px; top: 295.159px; font-size: 15px; font-family: serif; transform: scaleX(0.974771);" data-canvas-width="63.74999999999999">Key words</div><div style="left: 181.86px; top: 295.159px; font-size: 15px; font-family: serif; transform: scaleX(1.00343);" data-canvas-width="559.9949999999999">experimental osteomyelitis, patomorfologija, early diagnosis, mineral composition, osteopor</div><div style="left: 741.255px; top: 295.159px; font-size: 15px; font-family: serif; transform: scaleX(1.00029);" data-canvas-width="23.34">osis</div></div>experimental osteomyelitispatomorfologijaearly diagnosismineral compositionosteoporosisэкспериментальный остеомиелитпатоморфологияранняя диагностикаминеральный составостеопороз[1. 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