Evaluation of Antimicrobial Effect of Copper Nanoparticles’ and Low-intensity Laser Radiation and Their Combined Application in vitro Experiment

Relevance The study of nanoparticles’ bactericidal properties and laser radiation antimicrobial activity is quite relevant for experimental substantiation of laser nanotechnologies’ application efficiency. The purpose of the study To study the markedness of copper nanoparticles’ and low-intensity laser radiation (LILR) antimicrobial action and to evaluate the effectiveness of their combined application in in vitro experiment. Materials and methods Markedness of synthesized copper nanoparticles’ and laser radiation antimicrobial effect on Pseudomonas aeruginosa and Staphylococcus aureus was evaluated according to MacFarland by mixing daily cultures. In the first series of the experiments the culture of the microorganisms was subjected to radiation for two minutes using the ALT “Matrix” apparatus; in the second series copper nanopowder suspension was introduced into the culture of the microorganisms; in the third series laser radiation and introduction of copper nanoparticles were combined. Results and their discussion Low antibacterial LILR activity was noted; reliable decrease of the number of colonies was observed at nanocopper introduction. There was revealed reliable synergism of antimicrobial action of copper nanoparticles’ and LILR combined application. Conclusion Combined application of LILR and copper nanoparticles allows to obtain antibacterial effect at lower concentrations of copper nanoparticles, thus decreasing possible toxic action of the given substance on the organism in in vivo conditions.

Antimicrobial activity, low-intensity laser radiation, copper nanoparticles, combined application