Clinical-Morphologic Characteristics of Destructive Forms of Acute Pancreatitis in the Experiment by the Usage of Direct Current and Endovenous Ozone Therapy in Complex Treatment

Materials and methods The results of experimental study of destructive pancreatitis modeling which have carried out on 45 outbread dogs were analyzed in this thesis. Modeling of destructive pancreatitis in animals has been achieved via canalicular-hypertensive model by S.A.Shalimov. 4 series of experimental study were made to achieve the targeted goal. The first series 10 dogs without treatment, the second series 10 dogs in which conventional conservative therapy was used for the treatment of acute experimental destructive pancreatitis in animals, the third series 12 dogs that underwent intravenous ozone therapy in the complex together with medication therapy, in the forth series the effectiveness of combined administration of intravenous ozone therapy and small doses of direct current in 13 dogs was evaluated. Results and their discussion The animals of first series without treatment died after 1-3 days after modeling of disease. In pancreatic tissue after 1 day from the moment of modeling of destructive pancreatitis discovered augmentation of generation rate of hyperoxide outputs for 10%. Revealed the decreased activity of glutathione reductase (GR) in pancreatic tissue which achieves the critical level in the 3rd day (3 times lower than the basal value). In the second series of experiment died 6 dogs (60%) among 10 within the first week. In the third series in the process of experiment survived 8 animals from 12. Mortality 33,3%. In the forth series of the experiment by the 2 day was diagnosed the activity of glutathione reductase of pancreatic tissue, showings of which increased in 1,8 times at significant decrease of potency of glutamyltransferase in 2,2 times. Further showings of glutamine-dependent ferments stabilized and after 5 days didn’t differ from the initial data. The content of malondialdehyde in erythrocyte after 5 days decreased to 1,1 times more in comparison with control group. At the same time determined the decrease of malondialdehyde in pancreatic tissue – to 10 times in comparison with control group. In this series survived 10 animals from 13. Mortality 23%. Conclusion Therefore combined administration of small doses of DC and intravenous ozone therapy in the complex treatment shows antiphlogistic action, improves antioxidant function of organ, arrests the process of lipid peroxidation, favors accelerated rejection of necrotic tissue with the reduction of focus of inflammation, accelerating compensatory adaptive possibilities with activation of regeneration process in pancreas which allows to decrease mortality of experimental animals from 60% to 23%.

Постоянный электрический ток, озон, экспериментальное исследование