Clinical and morphological features of the wound healing process when managing simulated wounds with a sodium selenite and metronidazole-based medicinal product

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Abstract

Background. Currently, one of the major problems in surgery is the treatment of wounds and trophic ulcers. The relevance of this problem results from the fact that the concept of the wound process and its mechanisms are constantly changing due to the development of surgery and related disciplines.

Aims. The aim of this experimental study was to compare the clinical and morphological features of the wound-healing potential of the developed medicinal product in managing the first (I) and second (II) phases of the wound process.

Material and methods. An experimental study involved 36 male Wistar rats. In the study, we evaluated the effectiveness of the developed medicinal composition on wound healing. After wound simulation, local treatment with dressings was provided to animals. The animals were divided into three groups: the main, or experimental, group received a medicinal product created on the basis of sodium selenite, metronidazole, and dimethyl sulfoxide; the comparison group received a dimethyl sulfoxide-based ointment; and the control group received no wound treatment.

Results. It was found that in animals of the experimental group, the combination of dimethyl sulfoxide, metronidazole, and sodium selenite resulted in the decreased duration of wound healing phases, reducing the risk of reinfection. Selenium, in turn, due to its antioxidant properties, exerted a pronounced antioxidant effect at the site of the pathological lesion, significantly improving wound reparative processes, thereby reducing the second phase of the wound healing process. Wound healing in animals of the main group occurred in 8 days, compared to 11 days in the comparison group and 16 days in the control group.

Conclusions. Taking into account the data obtained, the authors concluded about the promising use of a medicinal composition with sodium selenite, metronidazole and dimethyl sulfoxide for the local treatment of wounds.

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About the authors

Evgeny V. Skvortsov

Clinical Hospital "RZD - Medicinе"

Author for correspondence.
Email: zh_scvortsov@mail.ru

Head of the Surgical Department

Russian Federation, Chita

Evgeny V. Namokonov

Chita State Medical Academy

Email: namokonovev@mail.ru

 M.D., Professor, Head of the Department of General and Specialized Surgery

Russian Federation, Chita

Nadezhda A. Shemyakina

Chita State Medical Academy

Email: shemyakina86@list.ru

Ph.D., Associate Professor of the Department of General and Specialized Surgery

Russian Federation, Chita

Tsyndyma B. Bayaskhalanova

Chita State Medical Academy

Email: bayasxalanovac@rambler.ru

Assistant of the Department of Histology, Embryology and Cytology

Russian Federation, Chita

Alexander M. Miromanov

Chita State Medical Academy

Email: miromanov_a@mail.ru

M.D., Professor, First Vice-Rector, Vice-Rector for Medical Work, Head of the Department of Traumatology and Orthopedics

Russian Federation, Chita

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Supplementary files

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2. Fig. 1. Magnification: 200x. Staining: hematoxylin-eosin. Control group on the third day: dense neutrophilic infiltration.

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3. Fig. 2. Magnification: 200x. Staining: hematoxylin-eosin. Control group on the sixth day: moderate neutrophilic infiltration, occasional macrophages, lymphocytes.

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4. Fig. 3. Magnification: 200x. Staining: hematoxylin-eosin. Comparison group on the third day: moderately expressed neutrophilic infiltration.

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5. Fig. 4. Magnification: 200x. Staining: hematoxylin-eosin. Comparison group on the 6th day: a thin leukocytic-necrotic layer and reduced infiltration. The granulation tissue contained lymphocytes, neutrophils, immature fibroblasts, newly formed blood vessels, and lymphostasis.

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6. Fig. 5. Magnification: 200x. Staining: hematoxylin-eosin. Main (experimental) group on the third day: moderately expressed neutrophilic infiltration, appearance of fibroblasts.

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7. Fig. 6. Magnification: 200x. Staining: hematoxylin-eosin. Main experimental group on the sixth day: most cells are fibroblastic cells and neutrophils.

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