postgraduate student of Department of the General surgery
Аспирант
MD, Head of Department of the General surgery
д. м. н., зав. кафедрой общей хирурги Омского государственного медицинского университета
MD, Honoured Scientist of the Russian Federation, Head of Department of the Pathophysiology including Clinical Pathophysiology Course of Omsk State Medical University
доктор медицинских наук, заслуженный деятель науки Российской Федерации, заведующий кафедрой патофизиологии Омского государственного медицинского университета
Background. At present time there are few works throwing some light on the details of solving a problem in a field of experimental medicine by the "how to do it" principle which after having studied them give readers a good grasp of reproducing the offered technique on their own. It also concerns blood sampling with the aim of assessing certain indicators at small laboratory animals.
Objectives. To work out the technique of carotid artery catheterization for detecting the referential meanings of biochemical and complete blood tests coagulogram on Wistar male rats. To define more precisely the obtained values by the bootstrap method and to compare them with the data to have published before.
Materials and methods. The research was conducted on Wistar male rats (n=42) weighing 270-380 g. The access to the left carotid artery was implemented with taking into account some anatomic features of an animal being anesthetized with tiletamin/zolazepam (20-40 mg/kg intramuscularly) + xylazine (5-10 mg / kg. intramuscularly). Statistical treatment has been conducted in the programming language R with using the statistical packages "boot", "car", "sm", "coin", "VIM" and "mice". The referential meanings have been defined by the bootstrap method.
Comparing with literature data was executed by the permutational test with p-level correction for multiple comparisons by using the Benjamini-Yekutieli method, the level α was accepted as equal 0,05.
Results and Discussion. The rational access to a carotid artery and its catheterization way have been developed. The referential meanings of biochemical and complete blood tests coagulogram were developed and defined by using the bootstrap method on Wistar male rats. While comparing the indicators to have received earlier from Wistar male rats by other researchers, the statistically significant differences were received in all indicators, except the quantity of leucocytes, thrombocytes, and indicators of hematocrit and total bilirubin. The received differences are likely to be connected with different climatic conditions, the character of anesthesia care, accessing to blood sampling, and wide range of the compared rats’ age. Clinically differences are insignificant.
Conclusion. The developed algorithm for obtaining referential meanings of blood tests samplings from rats can be considered to be rational and reproduced and the received indicators’ values should be considered to be referential.