Experimental Substantiation of Combined Copper Nanoparticles’ and Low-Intensity Laser Radiation Application in Surgical Treatment of Simulated Infected Burn Wounds of the Skin


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Abstract

Relevance Application of laser and nanotechnologies is considered to be one of the most current and promising trends in modern experimental medicine. The purpose of the study To develop the method of infected skin burn wound modelling and to substantiate experimentally the effectiveness of copper nanoparticles’ and low-intensity laser radiation (LILR) combined application in the treatment of infected burn wounds of the skin. Materials and methods On the basis of the experiment on 50 white laboratory rats of 190-200g. weight there was developed and patented the method of skin burn wound simulation; the effect of copper nanoparticles’ and low-intensity laser radiation combined application in the treatment of infected burn wounds was studied in the experiment on 50 similar animals Results and their discussion Using the developed laser radiation method, skin burns of controlled damage depth and area were modelled. Peculiarities of the simulated wound process development in skin burn wound infection, markedness of antibacterial effect of various copper nanoparticles’ concentrations and laser radiation parameters were detected. Combined local application of LILR and copper nanoparticles in experimental animals of the group under observation provided by the 7th day of treatment discontinuation of pathogenic microflora inoculation, by the 4th day – appearance of granulations, and by the 14th day of treatmentwound epithelization. Conclusion The obtained findings of experimental studies with the use of planimetric and microbiological methods of investigation suggest rather high efficiency of combined application of copper nanoparticles and laser radiation, the effect surpassing that of standard methods of treatment in duration and adequacy of wound epithelization

About the authors

V. I. Razumovsky Saratov State Medical University, 112 Bol'shaia Kazach'ia Str., Saratov, 410012, Russian Federation

Author for correspondence.
Email: vladimiralipov@yandex.ru
MD, Professor, Academician of the Russian Academy of Natural History, Head of the Department of Operative Surgery and Topographic Anatomy of Saratov State Medical University named after V.I. Razumovsky

V. I. Razumovsky Saratov State Medical University, 112 Bol'shaia Kazach'ia Str., Saratov, 410012, Russian Federation

Email: author@vestnik-surgery.com
Ph.D. Student of the Department of Operative Surgery and Topographic Anatomy of Saratov State Medical University named after V.I. Razumovsky

V. I. Razumovsky Saratov State Medical University, 112 Bol'shaia Kazach'ia Str., Saratov, 410012, Russian Federation

Email: author@vestnik-surgery.com
 External Doctoral Student of the Department of Operative Surgery and Topographic Anatomy of Saratov State Medical University named after V.I. Razumovsky

V. I. Razumovsky Saratov State Medical University, 112 Bol'shaia Kazach'ia Str., Saratov, 410012, Russian Federation

Email: author@vestnik-surgery.com
4th Year Student of Saratov State Medical University named after V.I. Razumovsky

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