Creation of Model of Acute Necrotizing Pancreatitis

Abstract


Relevance In the pathogenesis of acute pancreatitis remains a large number of unresolved issues. Therefore, experimental studies of ETA have not lost their relevance.
The purpose of the study Is developing of model of acute necrotizing pancreatitis (ANP) characterized by increased release of cytokines into peripheral blood.
Materials and methods In our research we had 60 12 to 14-week old male outbred (Sprague Dawley) SPF rats. Cerulein, alcohol and mechanical model were created.
Results and their discussion Cerulein injection at given doses did not induce pancreatic necrosis. This model was not associated with statistically significant elevation of cytokine levels. Alcohol model also did not cause pancreatic necrosis.
Conclusion Mechanical model was characterized by pronounced inflammation and necrosis of pancreatic tissue in all animals. The model of APN reproduced by creation of partial common biliary duct obstruction may be recommended for assessment of effectiveness of medications inhibiting cytokine release.

N.I. Pirogov Russian National Research Medical University, 1 Ostrovitianova Str., Moscow, 117997, Russian Federation

Author for correspondence.
Email: mail@vestnik-surgery.com
MD, Prof. of Department of Experimental and Clinical Surgery of N.I. Pirogov Russian National Research Medical University

N.I. Pirogov Russian National Research Medical University, 1 Ostrovitianova Str., Moscow, 117997, Russian Federation

Email: getinfo911@mail.ru
MD, associate prof. of Department of Experimental and Clinical Surgery of N.I. Pirogov Russian National Research Medical University

N.I. Pirogov Russian National Research Medical University, 1 Ostrovitianova Str., Moscow, 117997, Russian Federation

Email: mail@vestnik-surgery.com
Ph.D., associate prof. of Department of general pathology of N.I. Pirogov Russian National Research Medical University

Main Military Clinical Hospital of the Russian FSS, 48 Petrovskoe Rte., Golitsyno, 143040, Russian Federation

Email: mail@vestnik-surgery.com
surgeon in Main Military Clinical Hospital of the Russian FSS

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