The Germinal Epithelium of the Testis in Children with Cryptorchidism in the Ultrastructural Section

Abstract


Relevance. Male fertility is largely dependent on the timely testicular descent into the scrotum and the formation of the two major divisions of the testis: interstitial and seminiferous tubules.

The purpose of research - to reveal ultrastructural changes in the germinal epithelium of the testes with cryptorchidism and assess their degree of severity of patients of different age.

Materials and methods. To study the ultrastructure of testis in 30 children with inguinal retention testicular biopsy runtime relegated gonads were done. Before surgery clinically and sonographically determined testicle in the groin area. Children age 1 to 14 years. All the children with unilateral cryptorchidism. In the study of testicular tissue ultrastructural changes in cryptorchidism revealed 3 types of state tubules and germinal epithelium. Changes in type I revealed 4 cases (13%). In type II tubules in the testis revealed mixed cell type: along with light cells therein are determined cells with dark cytoplasm and signs of destruction - 13 boys (46.4%). In 11 (37%) boys identified type III tubules dominated cells with signs of degradation.

Conclusions. The study revealed destructive changes of the germinal epithelium of the seminiferous tubules with cryptorchidism: vacuolization of cytoplasm, cytoplasmic foci of local degradation of the matrix, swelling of mitochondria, cristae destruction and devastation of the mitochondrial matrix, pycnosis cores and peritubular interstitial testicular sclerosis, signs of microcirculation disorders.


Svetlana Yurievna Komarova

FSBEI НE Ural State Medical Uvinesity MOH Russia

Author for correspondence.
Email: urokom@yandex.ru
ORCID iD: 0000-0003-3570-3147
SPIN-code: 9411-6025

Russian Federation, 3 Repina str., Yekaterinburg, 620014, Russian Federation  51 Rusetskaya str., 51, Ekaterinburg, 620134, Russian Federation

Candidate of Medicine

Svetlana Vladimirovna Pichugova

Clinical-diaglostical cente Institute of Immunology and Physiology

Email: ekb-lem@mail.ru
SPIN-code: 8877-4258

Russian Federation, 115A Furmanov str., Ekaterinburg, 620144, Russian Federation 106 Pervomayskaya str., Yekaterinburg, 620049, Russian Federation

Doctor of the highest-grade electronic microscopy laboratory,

Candidate of Medical Sciences

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Copyright (c) 2017 Komarova S.Y., Pichugova S.V.

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