Peritonitis Model and Experimanetal Basis of Anti-Adhesion Therapy

Relevance Various methods of abdominal adhesive disease treatment are applied in clinical practice. In certain cases, they show low level of evidence on the impact on the forming collagen namely. The method of postoperative anti-adhesion therapy is developed and introduced into the Hospital of Pediatric Surgery (Patent for Invention No2348415 dated March 10th, 2009, Russia). Adhesion prevention should be started at the intraoperative period and prolonged to the period of adhesion formation, proved by morphologists. The purpose of the study Was to develop experimental peritonitis model and approve methods of intra- and postoperative antiadhesion therapy on its basis. Materials and methods 30 white laboratory male rats were involved into the study. 1-1.5mL of 1015 E.coli suspension was used for artificial peritoneal contamination. Contaminated rats developed peritonitis which was clinically close to human diffuse peritonitis and manifested in rapidly progressed intoxication, intestinal dysmotility, severe microcirculatory disorder. All the experimental animals were divided into 6 groups controlled for basic peritonitis model, drug used for sanitation of the abdominal cavity, and postoperative treatment. Intraoperative anti-adhesion therapy included sanitation with the Thiazole/Dioxidine solution, postoperative therapy included application of Thiazole/Lidase 128U composition on the anterior abdominal wall. Results and their discussion Experimental animals developed clinical symptoms of peritonitis in 30 minutes after the injection of infectious agent into the abdominal cavity. Rats from the groups I and II showed no pathological processes at revision of abdominal cavity. Rats from the groups III-VI manifested in severe signs of diffuse purulent peritonitis in one day after contamination. All the studying groups were monitored for severity and incidence rate of adhesions. Animals from the group V received staged Thiazole based anti-adhesion therapy and showed no adhesions. Conclusion Development of diffuse peritonitis model has made possible to carry out the experimental study on application of titanium glycerosolvate (Thiazole) aqua-complex in the treatment. Aqua-complex appeared to show high efficiency in sanitation of the abdominal cavity in combination with Dioxidine and inhibition of adhesion formation in combination with transcutaneous administration of Lidase.

Перитонит, противоспаечная терапия