Free-radical processes and antioxidant status in patients with enteral insufficiency


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Relevance Acute intestinal obstruction is accompanied by a violation of bowels haemocirculation, ischemia of the intestinal wall and enteral insufficiency, which leads to the activation of free radical processes. Quantitative evaluation of the expression of free radical processes and antioxidant status of the blood system in this pathology is particularly importance. The purpose of the study To determine the condition of free radical oxidation and antioxidant protection in blood serum, to estimate their predictive value, depending on the degree of enteral insufficiency for the postoperative period of patients with acute adhesive intestinal obstruction. Materials and methods 87 patients with acute adhesive intestinal obstruction, treated in a surgical department of SBHF "City Clinical Hospital № 1" in Orenburg in 2007-2012 were examined. All patients were operated emergency. Conservative treatment in the postoperative period in accordance with the current standard diagnostic and treatment was carried. The duration of postoperative intestinal paresis in all patients was estimated. In addition to standard clinical examination the leukocyte index of intoxication and the level of average weight molecules in blood serum was determined. Condition of free radical oxidation evaluated by determining the concentration of malondialdehyde and diene conjugates in blood serum, as well as the study of spontaneous chemiluminescence and iron-induced chemiluminescence of blood serum. Result and their discussion Leukocyte index of intoxication, the concentration of lipid peroxidation products and the average weight molecules in serum in the early postoperative period did not always correspond to the degree of intestinal dysfunction, and it can not predict the duration of postoperative intestinal paresis. Chemiluminescence indicators are informative predictor of enteral insufficiency syndrome in the preoperative and early postoperative period in patients with intestinal obstruction. Conclusion The syndrome of intestinal failure associated with activation of free radical processes and reduced antioxidant status. Indicators of chemiluminescence of blood serum are informative predictor of the development of enteral insufficiency syndrome as in the preoperative and early postoperative period in patients with intestinal obstruction.

About the authors

Orenburg State Medical Academy, Orenburg, Russian Federation

Author for correspondence.
Email: d.v.volkov@list.ru
PhD, associate prof. of the department of hospital surgery, urology of the Orenburg State Medical Academy

Orenburg State Medical Academy, Orenburg, Russian Federation

Email: k_gspsury@orgma.ru
MD, prof., the head of the department of hospital surgery, urology of the Orenburg State Medical Academy

Orenburg State Medical Academy, Orenburg, Russian Federation

Email: ks_oren@mail.ru
MD, prof., the head of the department of chemistry and pharmaceutical chemistry of the Orenburg State Medical Academy

Institute of Cellular and Intracellular Symbiosis UD of the RAS, Orenburg, Russian Federation

Email: sergfsb@rambler.ru
MD, the head of laboratory of microbial ecology of the Institute of Cellular and Intracellular Symbiosis of UD of the RAS, associate prof. of the department of hospital surgery, urology of the Orenburg State Medical Academy

Orenburg State Medical Academy, Orenburg, Russian Federation

Email: natalya.sharapova2010@yandex.ru
PhD, associate prof., of the department of chemistry and pharmaceutical chemistry of the Orenburg State Medical Academy

References

  1. Gain Iu.M., Leonovich S.I., Alekseev S.A. Sindrom
  2. enteral'noi nedostatochnosti pri peritonite: teoreticheskie
  3. i prakticheskie aspekty, diagnostika i lechenie [Enteral
  4. insufficiency syndrome in peritonitis: theoretical and
  5. practical aspects, diagnosis and treatment]. Minsk,
  6. Molodechno, 2000. 265 p. – (In Russian).
  7. Bagnenko S.F., Sinenchenko G.I., Kurygin A.A., Chupris
  8. V.G. Korrekciya reperfuzionnoj disfunkcii pri ostrom
  9. kishechnoj neprohodimosti [ The correction of reperfusion dysfunction in acute
  10. intestinal obstruction]. Vestnik khirurgii, 2008; 4: 32-35. –
  11. (In Russian).
  12. Gabrielian N.I. Diagnosticheskoe znachenie opredeleniya
  13. iz srednih molekul v plazme krovi v klinicheskoj
  14. nefrologicheskih zabolevanij [The diagnostic value of determination
  15. of the middle molecules in the blood plasma in clinical
  16. nephrological diseases]. Klinicheskaia meditsina, 1981; 10:
  17. -42. – (In Russian).
  18. Ermolov A.S., Popova T.S., Pakhomova G.V., Uteshev
  19. N.S. Sindrom kishechnoi nedostatochnosti v neotlozhnoiabdominal'noi khirurgii [The syndrome of intestinal
  20. failure in the emergency abdominal surgery]. Moscow,
  21. MedEkspertPress, 2005. 460 p. – (In Russian).
  22. Eriukhin I.A., Petrov V.P., Khanevich M.D. Kishechnaia
  23. neprokhodimost': rukovodstvo dlia vrachei [Intestinal
  24. obstruction: a guide for physicians]. Saint-Petersburg,
  25. «Piter» Publ., 1999. 448 p. – (In Russian).
  26. Kosinets V.A., Sachek M.G., Kondratenko G.G. Ispol'zovanie
  27. reamberinein v kompleksnoj terapii rasprostranennyj gnojnyj
  28. peritonit [The use of
  29. reamberinein in the complex therapy of widespread purulent
  30. peritonitis]. Khirurgiia, 2010; 1: 59-63. – (In Russian).
  31. Lang T.A., Sesik M. Kak opisyvat' statistiku v meditsine.
  32. Rukovodstvo dlia avtorov, redaktorov i retsenzentov.
  33. Moscow, Prakticheskaja medicina, 2011. 480 p. – (In
  34. Russian).
  35. Miliukov V.E., Sapin M.R. patogeneticheskie mekhanizmy
  36. razvitiya peritonita s ostroj tonkoj kishki
  37. neprohodimost' [Pathogenetic mechanisms
  38. of development of peritonitis with acute small intestine
  39. obstruction]. Khirurgiia, 2005; 7: 40–45. – (In Russian).
  40. Permiakov P.E., Zhidovinov A.A., Zurnadzh'iants V.A.,
  41. Krasilov V.L. Znachenie sindroma ishemii-reperfuzii
  42. v razvitii ehndogennoj intoksikacii s ostroj
  43. kishechnaya neprohodimost' [The value of ischemia-reperfusion syndrome
  44. in the development of endogenous intoxication with acute
  45. intestinal obstruction]. Infektsii v khirurgii, 2009; 2: 47-50.
  46. – (In Russian).
  47. Popova T.S., Tamazashvili T.Sh., Shestopalov A.E. Sindrom
  48. kishechnoi nedostatochnosti v khirurgii [The syndrome of
  49. intestinal failure in surgery]. Moscow, Medicina, 1991. 240
  50. p. – (In Russian).
  51. Puchkov K.V., Gausman B.Ia., Seliverstov D.V. Patogenez
  52. narushenij i metodov korrekcii regional'nogo
  53. gemodinamika s ishemiej [Pathogenesis
  54. of disturbances and methods for correcting regional
  55. hemodynamics with its ischemia]. Khirurgiia, 1997; 7: 64-
  56. – (In Russian).
  57. Rybachkov V.V. Kotiuchenko, K.V., Maevskii S.V. Peritonit
  58. [Peritonitis]. Jaroslavl', "IarMediaGrup" publ., 2010. 305 p.
  59. – (In Russian).
  60. Sirota T.V. Novyj podhod k izucheniyu processa
  61. avto-okisleniya adrenalina i ispol'zovat' ego dlya izmereniya
  62. aktivnost' superoksiddismutazy [A new approach to the study of the process
  63. of auto-oxidation of adrenaline and use it to measure the
  64. activity of superoxide dismutase]. Voprosy meditsinskoi
  65. khimii, 1999; 3: 56-58. – (In Russian).
  66. Stal'naia I.D. Sovremennye metody v biokhimii [Modern
  67. methods in biochemistry]. Moscow, 1977; 63-64. – (In
  68. Russian).
  69. Farkhutdinov R.R. Svobodnoradikal'noe okislenie v
  70. biologicheskom materiale i khemiliuminestsentnye
  71. metody issledovaniia v eksperimental'noi i klinicheskoi
  72. meditsine [Free radical oxidation in biological material and
  73. chemiluminescent methods of research in experimental and
  74. clinical medicine]. Ufa, 2002. 104 p. – (In Russian).
  75. Juvonen P.O., Paajanen H.E., Heino A.A., Koski E.J.,
  76. Merasto M., Hartikainen J.E., Kosma V.M., Alhava E.M.,
  77. Takala J.A. ZHirovye produkty perekisnogo i antioksidantom
  78. potenciala v portal'noj venoznoj i arterial'noj plazme sistemnoj
  79. pri postepennom kishechnoj ishemii i reperfuzii u svinej [Lipid peroxidation products and antioxidant
  80. capacity in portal venous and systemic arterial plasma
  81. during gradual intestinal ischemia and reperfusion in pigs].
  82. Eur. Surg. Res., 1998; Vol. 30: 2: 95-101.
  83. Mc Knight R.C., Hunter F.E. Sostav i fermentativnaya
  84. Meropriyatiya [Composition and enzymatic
  85. Activity]. J. Biol. Chem., 1966; 241: 12: 2757-2761.
  86. Modzelewski B. Produktov perekisnogo okisleniya lipidov v
  87. ehksperimental'nyj peritonit [Lipid peroxidation products in
  88. experimental peritonitis]. Pol. Merkur. Lekarski, 2005; 18:
  89. : 421-423.
  90. Zuck H. Metody fermentativnogo analiza [Methods of enzymatic analysis]. Ed. by Bergmeger
  91. H., Pergamon Press. 1963. 894 p.

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