Pathology and mineral composition of bone in the early stages of experimental osteomyelitis

Abstract


Relevance
Study of pathogenetic patterns and the nature of the structural changes in the bone tissue in the early stages of acute
osteomyelitis is relevant in terms of increased knowledge about mechanisms of development of complications in patients with
orthopedic and rehabilitation of the complex. The purpose of the work comprehensive assessment of pathological changes and
features of the mineral composition of bone tissue in the early stages of acute osteomyelitis in the experiment.
Materials and methods
The experiments were performed on 35 rabbits of both sexes, in accordance with bioethical requirements.
Acute hematogenous osteomyelitis modeled by introducing a culture of Staphylococcus aureus in the medullary canal of the tibia.
All animals were obtained from a short-term experience under ether anesthesia by air embolism after 30 min, 6, 12, 24 and 48 hours
after administration Staphylococcus aureus culture. Information on the timing of the experiment by light, electron (scanning and
transmission) microscopy and X-ray electron microprobe analysis studies especially pathomorphology and mineral composition of
bone tissue.
Results and their discussion
During the first 30 minutes of the experiment micro- and ultramicroscopic changes in bone were
minimal: preserved bone rods with small groups of red blood cells, and the mineral content of bones was characterized by a decrease
in the volume fraction of sodium. At the 6 hour experiment there was also no significant structural rearrangements of bone, but
there was a further reduction in the concentration of sodium and magnesium, and increase the volume fraction of sulfur. At the
same time, this period of the experiment was an increase in volume fraction of calcium and phosphorus. Morphological picture
in the next 12 hours of the experiment were characterized by the beginning of the destructive processes in the bone marrow, with
what is likely to involve some adjustment of mineral metabolism of bone tissue. There was a sharp increase in the concentration of
sodium while maintaining high values of volume fractions of intraosseous calcium, phosphorus and sulfur, reflecting the increased
mineralization. By the end of first day of the experiment to develop the process of destruction of bone tissue, which were accompanied
by demineralization. Second day from start of the experiment were activated processes of bone formation, and the demineralization
persisted.
Conclusions
Thus, within one day of the experiment structural rearrangements missing bone, and its mineral composition change
associated with the adaptation process active in response to inflammation in the medullary canal. Second day experience of developing
bone destruction is accompanied by phenomena of osteoporosis, along with activation of osteosynthesis. Period for first day from
entering the pathogen can be considered as the most favorable in terms of prevention of subsequent orthopedic complications.
Key words
experimental osteomyelitis, patomorfologija, early diagnosis, mineral composition, osteopor
osis

Izhevsk State Medical Academy, 426034, Izhevsk, Udmurt republic, Kommunarov Street, 281

Author for correspondence.
Email: mail@vestnik-surgery.com
д.м.н., проф., ректор Ижевской государственной медицинской академии

Izhevsk State Medical Academy, 426034, Izhevsk, Udmurt republic, Kommunarov Street, 281

Email: mail@vestnik-surgery.com
д.м.н., проф., зав. кафедрой патологической анатомии Ижевской 

государственной медицинской академии

Izhevsk State Medical Academy, 426034, Izhevsk, Udmurt republic, Kommunarov Street, 281

Email: medic_82@mail.ru
аспирант кафедры хирургических болезней детского возраста Ижевской государственной медицинской академии

  1. V.A. Tarakanov, V.M. Nadgeriev, V.M. Starchenko, A.E.
  2. Stryukovskiy, A.N. Lunyaka, E.G. Kolesnikov, N.K. Barova. Optimal'nye kriterii ranney diagnostiki i lecheniya ostrogo gematogennogo osteomielita u detey [Optimum criteria for early diagnostics and treatment of children's acute hematogenous osteomyelitis. Kuban scientific medical herald]. 2013; 7: 118-120 (in Russ.).
  3. S.N. Gisak, A.A. Shestakov, D.A. Baranov, E.A. Sklyarova. Sovremennye osobennosti etiopatogeneza ostrogo gematogennogo osteomielita u detey i optimizatsiya lecheniya bol'nykh. Vestnik novykh meditsinskikh tekhnologiy [Modern particulars for children's aetiopathogenesis of acute hematogenous osteomyelitis and optimization of patient treatment. Herald of new health technology]. 2012; ХIХ: 2: 106-108 (in Russ.).
  4. Strelkov, N.S. Patogeneticheskie metody ranney diagnostiki i lecheniya ostrogo gematogennogo osteomielita u detey: avtoref. dis. ... d-ra med. nauk[Pathogenetic methods for early diagnostics and treatment of children's acute hematogenous osteomyelitis. Abstract Diss. Doc. Med.]. 1999; 23 (in Russ.) .
  5. Bouvier, M. In vitro mineralization of a three-dimensional collagen matrix by human dental pulp cells in the presence of chondroitin sulphate. М. Bouvier, А. Joffre, Н. Magloire. Arch Oral Biol. 1990; 35: 4: 301-309.
  6. Lew, D.P. Osteomyelitis. D.P. Lew, F.A. Waldvogel. Lancet. 2004; 364: 369-379.
  7. Mustafa, M. Osteomyelitis: pathogenesis, clinical and therapeutic challenge. M. Mustafa, S.M. Yusof, M. Iftikhar. International Journal of Medicine and Pharmaceutical Sciences. 2014; 4: 9-18.
  8. Pathologic fractures in children with acute Staphylococcus aureus osteomyelitis. M.V. Belthur, S.B. Birchansky, A.A. Verdugo, E.O. Jr. Mason, K.G. Hulten, S.L. Kaplan, E.O. Smith, W.A. Phillips, J. Weinberg. J. Bone Joint Surg. Am. 2012; 94: 1: 34-42.
  9. Song, K.M. Acute Hematogenous Osteomyelitis in Children.
  10. K.M. Song, J.F. Sloboda. Journal of the American Academy of
  11. Orthopaedic Surgeons. 2001; 9: 3: 34-42.

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