Experimental Substantiation of a New Method of Treatment of Cerebral Ischemia in Rats


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Abstract

Currently installed neuroprotective properties of erythropoietin(EP)associated with anti-apoptotic and antihypoxic action. Along with this, it is known that the influence of the laser radiation in the middle infrared range increases microcirculation, increase the functional activity of endothelial cells that leads to activation of neoangiogenesis. The purpose of the study To develop a method of treating ischemia in the cerebral cortex(GM) on the basis of studies of behavioral and vascular reactions, pathological changes in animals with the introduction of recombinant EP and the influence of the laser radiation. Materials and methods An experiment was conducted at 130 outbred adult rats of both sexes weighing 220-250g. Animals are divided into 5 series of experiments: 1-series - modeling cerebral cortex GM (n=30). 2-I series - ischemia crust GM and the introduction of intraperitoneal recombinant EP (n=30). 3 series - ischemia crust GM and cutaneous laser irradiation region of the ischemic lesion(n=30. 4-series - ischemia crust GM treated with EP in conjunction with cutaneous laser irradiation(n=30). 5 series(control) rats with craniotomy, but without modeling the cerebral cortex GM (n=10). Animals were taken from experiment 7, 14, 30 days. Results and their discussion It is shown that the introduction of recombinant EP in combination with laser radiation in cerebral cortex GM increases in the early stages of the experiment, the tolerance of neurons to hypoxic damage, improves microcirculation. This is probably due to anti-apoptotic and antihypoxic action EP, and increased functional activity of endothelial cells under laser irradiation. Сonclusion 1. The combined effect of recombinant EP and laser radiation significantly reduces the area of the heart, enhances proliferation of endothelial cells with the development of new vasculature in the early stages of the experiments. 2. These structural changes are accompanied by an earlier regression of neurological disorders and improving behavioral reactions in experimental animals

About the authors

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Author for correspondence.
Email: author@vestnik-surgery.com
– MD, Prof., Vice Director, State Budget Institution of Health Center to provide specialized medical care, "Chelyabinsk State Institute of Laser Surgery" Russian Federation

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Email: author@vestnik-surgery.com
- MD, Prof., Director, State Budget Institution of Health Center to provide specialized medical care, "Chelyabinsk State Institute of Laser Surgery"; Russian Federation

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Email: ak_n@rambler.ru
Chairman of the Department of Neurosurgery, State Budget Institution of Health Regional Clinical Hospital №3 Russian Federation

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Email: author@vestnik-surgery.com
MD, Prof., worked at the State Budget Institution of Health Center to provide specialized medical care, "Chelyabinsk State Institute of Laser Surgery" Russian Federation

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Email: author@vestnik-surgery.com
Ph.D., Head of the Department of the Scientific Research, State Budget Institution of Health Center to provide specialized medical care, "Chelyabinsk State Institute of Laser Surgery" Russian Federation

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Email: author@vestnik-surgery.com
Neurosurgeon, Department of Neurosurgery, State Budget Institution of Health Regional Clinical Hospital №3; Russian Federation

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Email: author@vestnik-surgery.com
Ph.D., Senior Scientific Researcher, Division of Engineering and Physics, State Budget Institution of Health Center to provide specialized medical care, "Chelyabinsk State Institute of Laser Surgery"; Russian Federation

Cheliabinsk State Institute of Laser Surgery, 287 Pobedy Ave., Cheliabinsk, 454021, Russian Federation

Email: author@vestnik-surgery.com
– Ph.D., Senior Scientific Researcher, Division of Engineering and Physics, State Budget Institution of Health Center to provide specialized medical care, "Chelyabinsk State Institute of Laser Surgery" Russian Federation

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