The Role of Microsomal-Monooxygenase System of Liver and Indirect Electrochemical Oxidation of Blood in the Formation Mechanism of Endogenous Intoxication Syndrome in Animals with Experimental Bile Peritonitis

Abstract


Relevance On the background of growth in the number of patients JCB problem ha is of particular relevance. Ha leads to the development of systemic inflammatory response, which is accompanied by excessive generation of cytokines, are able to inhibit the activity of cytochrome P-450-dependent MOS liver, responsible for the biotransformation of endogenous compounds. The purpose of the study Is to evaluate the modulating effect NGH on MOS liver in experimental. Materials and methods Study of cytochrome P-450-dependent MOS liver conducted on 120 mongrel rats-males, weighing 160-200, by the method of A.I. Archakov (1975) and I.I. Carosino et al., (1977). The protein content in a fraction of microsomes determined by O. Lowry (1951). The number of microsomal cytochrome P-450 were determined by the method of T. Omura, R. Sato (1964). State MOS liver was estimated by the rate N-demethylation of antipyrine and gidrauxilirovania aniline (I.I. Karuzina, A.I. Archakov, 1977). Results and their discussion Experiments indicate the development of the syndrome of EI in animals with ha oppression antitoxic function of the liver. Introduction of 0.03% solution NGH causes a significant increase of cytochrome P-450 and b5, indicating that it permantently activity, at the same time as the use of 0.1% solution NGH causes a decrease of their content, indicating fermentarii effect. Conclusion Obtained result is of considerable clinical importance, because the biotransformation of many of the antibiotics used in the treatment of acute abdominal pathology occurs almost exclusively microsomal enzymes hepatocytes that against bacterial infection there is a significant risk of overdose, therefore, the use of 0.03% solution NGH can contribute to the induction of cytochrome P450 liver

Kuban State Medical University, 4 Sedina Str., Krasnodar, 350063, Russian Federation

Author for correspondence.
Email: author@vestnik-surgery.com

Russian Federation postgraduate of the department of operative surgery and topographic anatomy of the Kuban State Medical University.

Kuban State Medical University, 4 Sedina Str., Krasnodar, 350063, Russian Federation

Email: author@vestnik-surgery.com

Russian Federation PhD, assistant of the department of operative surgery and topographic anatomy, State educational state-funded institution of higher professional education of the Kuban State Medical University.

Kuban State Medical University, 4 Sedina Str., Krasnodar, 350063, Russian Federation

Email: superego_ksmu@mail.ru

Russian Federation MD, Professor of operative surgery and topographical anatomy, State educational state-funded institution of higher professional education of the Kuban State Medical University. E-mail: superego_ksmu@mail.ru.

  1. Archakov A. I. Mikrosomal'nogo okisleniya. [Microsomal oxidation.] M.:Nauka, 1975; p. 327
  2. Botashev A. A., the Landlord, Yu., Tereshchenko O. A., etc. Rol' markerov disfunkcii ehndoteliya sosudov pri abdominal'nom sepsise zhelchnogo proiskhozhdeniya. Infekcii v hirurgii. [the Role of markers of endothelial dysfunction of the vessels in the abdominal sepsis biliary origin. Infection in surgery.] 2012; 4: 6-10.
  3. Gallinger Yu. I. Laparoskopicheskaya holecistehktomiya: opyt 3165 operacij. EHndoskopicheskoj hirurgii. [Laparoscopic cholecystectomy: experience 3165 operations. Endoscopic surgery.] 2007; 2: 3-7.
  4. In Ivashkin.T., Buyeverov A. O., Lapina T. L. Gastroehnterologiya novogo veka: problemy diagnostiki. Terapevticheskij arhiv. [Gastroenterology of the new century: problems of diagnosis. Therapeutic archive.] 2001; 8: 33-36.
  5. Kanaeva I. P., Petushkova N. And., Lokhov P. G., et al. a Issledovanie mikrosom kletok pecheni myshi metodami proteomnogo analiza. Biomed. [Study of the microsomes of liver cells mouse proteomic methods of analysis. Biomed.] 2004; 4: 367-375.
  6. In Kukes., Sychev D., Shih, E. Izuchenie biotransformacii lekarstvennyh preparatov put' k povysheniyu ehffektivnosti i bezopasnosti farmakoterapii. Doktor. [a Study of the biotransformation of pharmaceuticals a way to improve the efficiency and safety of pharmacotherapy. Doctor.] 2007; 1: 6-8.
  7. Luzhniki E. A., Goldfarb Yu. S., Ostapenko Yu. N. Klinicheskaya toksikologiya na rubezhe XXI veka. Anesteziologiya i reanimatologiya. [Clinical toxicology at the turn of the twenty-first century. Anesthesiology and intensive care.] 1999; 6: 67-70.
  8. Novozheev T. P., Saratikov A. S. Vliyanie benzonala, kalanala i galadima na razvitie postishemicheskih rasstrojstv pecheni u krys. Himiko-farmacevticheskij zhurnal. [Effect of benzonal, kalanala and galadima on the development of postischemic disorders of the liver in rats. Chemical-pharmaceutical journal.] 2003; 12: 3-4.
  9. Novozheev T. P., Smagina M. I., Cherevko N. And., Fateev S. N. Fermentabilit i Pentobarbital – fenobarbital Tip induktory monooksigenaznoj sistemy pecheni. Byulleten' Sibirskoj mediciny. [Fermentability and Pentobarbital – phenobarbital type inductors of the monooxygenase system of the liver. Bulletin of the Siberian medicine.] 2011; 5: 79-81.
  10. Olisov O. D., Kubyshkin V. A. travma zhelchnyh protokov i ee posledstviya. Annaly hirurgicheskoj gepatologii. [bile duct Injury and its consequences. The annals of surgical Hepatology.] 2005; 10: 113-121.
  11. Petrosyan E. A., Tereshchenko O. A., A. A. Botashev etc. vliyanie gipohlorita natriya na nekotorye chasti gomeostaza pri lechenii ehksperimental'nogo zhelchnogo peritonita. Fundamental'nye issledovaniya. [the Effect of sodium hypochlorite on some parts of homeostasis in the treatment of experimental biliary peritonitis. Basic research.] 2010; 11: 98-103.
  12. Petrosyan E. A. Sergienko.And., Kade A. Kh., etc. Sposob
  13. modelirovaniya zhelchnogo peritonita.[Method
  14. modeling bile peritonitis.] RF patent № 2175784 from 10.11.2001., Bull.2001, No. 31.
  15. In Sergienko.And., Petrosyan E. A., Botashev A. A. and others EHndotelial'naya disfunkciya i sposoby ee korrekcii pri
  16. ehksperimental'nym zhelchnym peritonitom. Hirurgii. [Endothelial dysfunction and methods of its correction at
  17. experimental bile peritonitis. Surgery.] 2012; 3: 54-58.
  18. In Chernov.N., Belik B. M., Pshukov H. S. Rezul'taty prognozirovaniya i vybor hirurgicheskoj taktiki pri rasprostranennom gnojnom peritonite. Hirurgii. [Prediction outcome and choice of surgical tactics in widespread purulent peritonitis. Surgery.] 2004; 3: 47-50.
  19. Conney A. H. Pharmacoloical posledstviya mikrosomal'noj fermentnoj indukcii. [Pharmacoloical implications of microsomal enzyme induction.] Pharmacol. Rev. 1967; 19: 317-366.
  20. Harbrecht B., fry, R., Zenati, M. citohrom R-450 aktivnost' differencirovanno izmenyaetsya v osobo tyazhelyh pacientov. Krit. Uhod Med. [cytochrome P-450 activity is differentially altered in severely injured patients. Crete. Care Med.] 2005; 33: 3: 541-546.
  21. Hung, D., Siebert G., Chan P. et al. Pecheni farmakokinetika propranolola u krys s ad"yuvant-inducirovannogo sistemnogo vospaleniya / / Vestn. AM. ZH. Fiziologiya. Nauk. Gastrointest. Fiziologiya Pecheni. [Hepatic pharmacokinetics of propranolol in rats with adjuvant-induced systemic inflammation / / Vestn. AM. J. Physiology. Sciences. Gastrointest. Liver Physiol.] 2006; 290: 2: 343-351.
  22. S. B. Kang, N.-S. Han, min C. K., Lee H. K. Netravmaticheskie perforaciya zhelchnyh protokov u vzroslyh. [Nontraumatic perforation of the bile duct in adults.] Arch. Surg. 2004; 139: 1083-1087.
  23. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Izmerenie protein s Reaktiv fenol Polina. Dzhon. Biol. Him. [protein measurement with phenol reagent Polina. John. Biol. Chem.] 1951; 193; 1: 265-275.
  24. Tip: Y., Takemura, S., Yamasaki, K. et al. Postoyannyj priem organicheskih nitratov umen'shaetsya pechenochnogo citohroma R-450 [Continuous administration of organic nitrate decreases hepatic cytochrome P-450.] In J. Pharmacol. Exp. There. 2004; 308: 2: 729-735.
  25. Morgan E. regulirovanie citohroma R-450 vo vremya podverzheniya k vospaleniyu i infekcii. [regulation of cytochrome P-450 during exposure to inflammation and infection.] Drug Metab. Rev. 1997; 29: 1129-1188.
  26. Nebert D. Russell D. Klinicheskoe znachenie citohromov R-450. [clinical importance of the cytochromes P-450.] Lancet, 2002; 360: 9340: 1155-1162.
  27. T. Omura, R. Sato, Oksid ugleroda pigmenta sekvestr v mikrosomah pecheni 11. Ochistka i svojstva solyubilizacii. [oxide-carbon sequestration pigment in liver microsomes 11. Purification and solubilization properties.] John. Biol. Chem., 1964; 239: 2378-2385.
  28. Renton K. Regulyaciya metabolizma preparata i rasporyazheniya vo vremya podverzheniya k vospaleniyu i infekcii. EHkspert. OPIN. [regulation of drug metabolism and disposition during exposure to inflammation and infection. The expert. OPIN.] Drug Metab. Toxicol., 2005; 1: 4: 629-640.
  29. Tanaka E., Breimer D. V estestvennyh usloviyah testy funkcii pecheni preparat-okislitel'noj sposobnosti u pacientov s bolezn'yu pecheni. [in vivo function tests of hepatic drug-oxidizing capacity in patients with liver disease.] Z. Klin. Pharm. There., 1997; 22: 4: 237-249.

Views

Abstract - 63

PDF (Russian) - 171

PlumX

Dimensions


Copyright (c) 2014 ., ., .

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies