The Role of Microsomal-Monooxygenase System of Liver and Indirect Electrochemical Oxidation of Blood in the Formation Mechanism of Endogenous Intoxication Syndrome in Animals with Experimental Bile Peritonitis


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Abstract

Relevance On the background of growth in the number of patients JCB problem ha is of particular relevance. Ha leads to the development of systemic inflammatory response, which is accompanied by excessive generation of cytokines, are able to inhibit the activity of cytochrome P-450-dependent MOS liver, responsible for the biotransformation of endogenous compounds. The purpose of the study Is to evaluate the modulating effect NGH on MOS liver in experimental. Materials and methods Study of cytochrome P-450-dependent MOS liver conducted on 120 mongrel rats-males, weighing 160-200, by the method of A.I. Archakov (1975) and I.I. Carosino et al., (1977). The protein content in a fraction of microsomes determined by O. Lowry (1951). The number of microsomal cytochrome P-450 were determined by the method of T. Omura, R. Sato (1964). State MOS liver was estimated by the rate N-demethylation of antipyrine and gidrauxilirovania aniline (I.I. Karuzina, A.I. Archakov, 1977). Results and their discussion Experiments indicate the development of the syndrome of EI in animals with ha oppression antitoxic function of the liver. Introduction of 0.03% solution NGH causes a significant increase of cytochrome P-450 and b5, indicating that it permantently activity, at the same time as the use of 0.1% solution NGH causes a decrease of their content, indicating fermentarii effect. Conclusion Obtained result is of considerable clinical importance, because the biotransformation of many of the antibiotics used in the treatment of acute abdominal pathology occurs almost exclusively microsomal enzymes hepatocytes that against bacterial infection there is a significant risk of overdose, therefore, the use of 0.03% solution NGH can contribute to the induction of cytochrome P450 liver

About the authors

Kuban State Medical University, 4 Sedina Str., Krasnodar, 350063, Russian Federation

Author for correspondence.
Email: author@vestnik-surgery.com
postgraduate of the department of operative surgery and topographic anatomy of the Kuban State Medical University. Russian Federation

Kuban State Medical University, 4 Sedina Str., Krasnodar, 350063, Russian Federation

Email: author@vestnik-surgery.com
PhD, assistant of the department of operative surgery and topographic anatomy, State educational state-funded institution of higher professional education of the Kuban State Medical University. Russian Federation

Kuban State Medical University, 4 Sedina Str., Krasnodar, 350063, Russian Federation

Email: superego_ksmu@mail.ru
MD, Professor of operative surgery and topographical anatomy, State educational state-funded institution of higher professional education of the Kuban State Medical University. E-mail: superego_ksmu@mail.ru. Russian Federation

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