Metabolic correction of the ischemic-reperfusive liver damage against the background of its vascular exclusion in experimental conditions

It is an urgent issue of practical hepatology to find and to use means possessing the tissue protective properties to increase the potential of the own antioxidant system in conditions of the vascular liver exclusion which have to satisfy the practical medicine.

The purpose of the present study is to research the influence of intraperitoneal injections of the sodium dichloroacetate on the course of the ischemic-reperfusive syndrome in conditions of the modeled vascular liver exclusion.

Materials and methods. The study has been performed on 105 non-linear male rats divided into 7 groups. After the laparotomy animals have undergone the intraperitoneal injection of the sodium dichloroacetate in dosage 300 mg/kg under the general anesthesia, then the hepatoduodenal ligament has been clamped for 10, 15 and 20 minutes. The comparison groups have been formed from animals that have undergone the same manipulations without injections of the sodium dichloroacetate. The control group has been made up of 15 rats only laparotomy has been performed on. After 15 minutes of reperfusion the blood sampling from the caudal vena cava and liver has been carried out to perform further laboratory analysis. In the blood plasma the activity of lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase has been determined as well as the content of general thiol groups. In erythrocytes and liver homogenate the activity of catalase and superoxide dismutase have been determined. To evaluate the endotoxicosis level the content of substances of medium and low molecular mass in erythrocytes and the blood plasma has been studied.

Results and discussion. It has been revealed during the performed study that the sodium dichloroacetate significantly decreases the manifestation of the cytolytic syndrome. The increase of transaminase activity in the blood plasma by injection of the sodium dichloroacetate has fallen behind for 5 minutes in average in comparison with the groups without metabolic correction. The activity of lactate dehydrogenase in the blood plasma has been lower than the indices of the comparison groups by 2-3 times. The activity of enzymes of antiradical protection by injections of the sodium dichloroacetate has increased both in erythrocytes and the liver homogenate against the background of the lowering in the comparison groups. The content of thiol groups in the blood plasma within 10-20 minutes of the vascular exclusion has decreased by 25-38% and has persisted on this level while in the comparison groups the concentration decrease by 51.2% has been revealed to the 20^th minute. By the correction the significantly lower level of endotoxicosis has been developing. One of the possible mechanisms of cytoprotective action of the sodium dichloroacetate is the activation of antioxidant system due to the development of oxidative stress as the leading damage factor by reperfusion.

Conclusion. The received data demonstrate the cytoprotective properties of the sodium dichloroacetate in conditions of the developing ischemic-reperfusive syndrome on the model of the vascular liver exclusion in experimental conditions. The results of the study perspectively allow expanding the usage possibilities of the vascular liver exclusion in its various types in the field of surgical hepatology.